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Ion Exchange Chromatography: History, Principles, and Real‑World Applications

Ion exchange chromatography (IEX) is a liquid chromatography technique that separates molecules by their charge using a charged stationary phase and an aqueous mobile phase. It is a workhorse for purifying proteins, nucleic acids, and inorganic ions in both research and industry .​ Brief history Early observations of ion exchange date back to 19th‑century soil …

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